Researchers have identified a protein that could be targeted to prevent transmission of the dengue virus, an advance that could ead to the development of a vaccine against the deadly in ection. An estimated 2 billion people are at risk for being bitten by Aedes mosquitoes and nfected with the dengue virus (DENV).
Researchers have found a candidate target for a transmission-blocking vaccine that interferes with virus in section of the mosquito after it feeds on the blood of infected hosts. Researchers from the University of South Caro ina and Central Michigan University, studied mosquito genes up-regulated during DENV infection as some of them are likely required for virus survival or infection.
Having previously identified a number of such genes, they here focused on one of them -which they termed CRVP379 -that codes or a putative cysteine-rich venom protein. The researchers found that CRVP379 is required during DENV in section in mosquito cells and in live mosquitoes, and that there is a direct correlation between the amount of CRVP379 expressed in the mosquito gut (where infection initiates) and the level of DENV infection in the gut and in whole mosquitoes.
They went on to show that CRVP379 interacts with a protein called prohibitin that is a putative DENV receptor in mosquitoes. When the researchers fed Aedes mosquitoes antibodies able to recognise CRVP379, potentially blocking the interaction of the protein with DENV, they found that this inhibits DENV infection of the mosquitoes. "These results further our understanding of DENV pathogenesis in the mosquito vector and highlight a potential target protein for the creation of a DENV transmission-blocking vaccine to break the hostvector transmission cycle," the researchers said
Researchers have found a candidate target for a transmission-blocking vaccine that interferes with virus in section of the mosquito after it feeds on the blood of infected hosts. Researchers from the University of South Caro ina and Central Michigan University, studied mosquito genes up-regulated during DENV infection as some of them are likely required for virus survival or infection.
Having previously identified a number of such genes, they here focused on one of them -which they termed CRVP379 -that codes or a putative cysteine-rich venom protein. The researchers found that CRVP379 is required during DENV in section in mosquito cells and in live mosquitoes, and that there is a direct correlation between the amount of CRVP379 expressed in the mosquito gut (where infection initiates) and the level of DENV infection in the gut and in whole mosquitoes.
They went on to show that CRVP379 interacts with a protein called prohibitin that is a putative DENV receptor in mosquitoes. When the researchers fed Aedes mosquitoes antibodies able to recognise CRVP379, potentially blocking the interaction of the protein with DENV, they found that this inhibits DENV infection of the mosquitoes. "These results further our understanding of DENV pathogenesis in the mosquito vector and highlight a potential target protein for the creation of a DENV transmission-blocking vaccine to break the hostvector transmission cycle," the researchers said
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